77 - Noval near infrared pH fluorescent sensor

Xiaole Shao¹, lovecindy85@gmail.com, Timothy E Glass¹, Mark A Milanick². (1) Department of Chemistry, University of Missouri- Columbia, Columbia, MO 65211, United States, (2) Department of Medical Pharmacology and Physiology, University of Missouri- Columbia, Columbia, MO 65211, United States

Noninvasive measurement of blood analytes would have a great impact on disease monitoring in human health, since blood composition reveals key details about a patient's well-being. Many fluorescent sensors exist to measure such blood parameters, however, due to the strong fluorescence background of serum (300-650 nm), such sensors would ideally emit in the near infrared (NIR). Such sensors typically would have low circulating life times.
Mammalian red blood cells (RBC) have no organelles, no nucleus, high glutathione levels, and essentially no protein synthesis, thus their cytosolic milieu provides an ideal environment for sensor stability. Furthermore RBCs have a circulating lifetime of roughly 120 days. A system for encapsulating NIR fluorescent sensors inside of RBC 'ghosts' will be presented. A NIR- pH sensor inside of RBC ghosts has been prepared and characterized. Additionally, NIR sensors for other important blood analytes will be presented.

Sunday, August 19, 2012 02:40 PM
Materials for Health and Medicine (01:00 PM - 04:40 PM)
Location: Pennsylvania Convention Center
Room: 201 C