PHYS Mark Johnson  Wednesday, March 24, 2010 

291 - Comparison of the enzymatic and spectroscopic properties of DNA photolyase and Cry-DASH enzymes

Maire Newton1, newtonm@lafayette.edu, Johannes P.M. Schelvis2, Yvonne M Gindt1. (1) Department of Chemistry, Lafayette College, Easton, PA 18042, United States, (2) Department of Chemistry, Montclair State University, Montclair, NJ 07043, United States

Cyclobutane pyrimidine dimers (CPDs) form in DNA upon illumination with UV light, and these lesions lead to cell mutation and, potentially, cell death. DNA photolyase and cryptochrome-DASH are two enzymes in the blue-light photoreceptor family that are able to repair CPDs in DNA. While both enzymes have been reported to repair single-stranded DNA, only DNA photolyase has been reported to repair double-stranded DNA. We analyze the enzymatic activity, thermodynamics, and spectroscopic properties of both DNA photolyase and cryptochrome-DASH to better characterize each enzyme and understand the mechanism by which each protein recognizes, binds, and releases DNA substrate.


Wednesday, March 24, 2010 06:00 PM
Fluorescent Probes (06:00 PM - 08:00 PM)
Location: The Moscone Center
Room: Hall D

 

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